The influences of SCN3A gene polymorphism on the efficacy of valproic acid sodium in the treatment of epilepsies / 中华行为医学与脑科学杂志

Objective:To investigate the influences of SCN3A gene polymorphism(c.905A>G/p.N302S and c. 1441C>T/p.L481L) on the efficacy of valproic acid sodium in the treatment of Zhuangzu epilepsies.Methods:Using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP)technique and the way of direct sequence, the SCN3A gene c. 905A>G/p.N302S and c. 1441C>T/p.L481L genotypes in peripheral blood were detected in 244 epileptic patients (85 cases in effective group and 139 cases of ineffective group) in the standardized treatment of valproic acid sodium.The blood concentration of valproic acid sodium was detected by LC-MS.Evaluating the correlation between the genotype and alleles of two groups of patients and the efficacy of valproic acid sodium and analyzing the difference of valproic acid sodium's blood concentration between different genotypes.The linkage disequilibrium of c. 905A>G/p.N302S and c. 1441C>T/p.L481L were analyzed by software SHEsis.Results:The allele and genotype distribution in c. 905A>G/p.N302S loci between effective group(A, G allele: 50.6%, 49.4%, AA, AG, GG genotype: 27.1%, 47.1%, 25.8%) and ineffective group(A, G allele: 37.4%, 62.6%, AA, AG, GG genotype: 16.6%, 41.7%, 41.7%) had statistically significant difference(χ 2=7.501, P=0.006; χ 2=7.907, P=0.019). There was no significant difference in allele and genotype distribution of c. 1441C>T/p.L481L loci between effective group(C, T allele: 47.1%, 52.9%, CC, CT, TT genotype: 23.5%, 47.1%, 29.4%) and ineffective group(C, T allele: 38.8%, 61.2%, CC, CT, TT genotype: 18.7%, 40.3%, 41.0%)(χ 2=2.920, P=0.088; χ 2=3.099, P=0.212). Compared with the AA + AG genotype, the GG genotype at c. 905A>G/p.N302S locus significantly reduced the efficacy of valproic acid sodium ( OR=2.051, 95% CI=1.136-3.703). Compared with genotypes AA+ AG, there were no significant differences in blood concentration of genotype GG of c. 905A>G/p.N302S ( t=3.256, P=0.137). Compared with genotypes CC+ CT, there were no significant differences in blood concentration of genotype TT of c. 1441C>T/p.L481L( t=4.628, P=0.082). c.905A>G/p.N302S and c. 1441C>T/p.L481L were without linkage disequilibrium. Conclusion:These results suggest that the single nucleotide polymorphisms of c. 905A>G/p.N302S in SCN3A genes may play a role in the resistivity of valproic acid sodium in Zhuangzu epilepsies.

Association of single nucleotide polymorphisms of SCN1A gene with therapeutic effect of carbamazepine among ethnic Zhuang Chinese patients with epilepsy / 中华医学遗传学杂志

OBJECTIVE@#To assess the association of single nucleotide polymorphisms of SCN1A gene with therapeutic effect of carbamazepine among ethnic Zhuang Chinese patients with epilepsy.@*METHODS@#Peripheral blood samples were taken from 186 epileptic patients for whom 66 cases standard regime of carbamazepine treatment was effective. Genotypes of rs3812718 and rs1813502 loci of the SCN1A gene were determined by Mass ARRAY-IPLEX and matrix assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS). Correlation between genotypes of patients and efficacy of carbamazepine treatment was analyzed.@*RESULTS@#Three genotypes (GG, GA and AA) were detected at both rs3812718 and rs1813502 loci of the SCN1A gene. A significant difference was found in allelic distribution (chi-square=17.810, P=0.000) and genotypic distribution (chi-square=17.873, P=0.000) of the rs3812718 locus between the effective group and ineffective group. No such difference was found with the rs1813502 locus (chi-square=1.606, P=0.206; chi-square=1.546, P=0.462, respectively). Compared with the GG+GA genotype, the AA genotype at rs3812718 locus significantly reduced the antiepileptic efficacy of carbamazepine (OR=3.776, 95%CI: 2.007-7.105). Among the 66 patients who were responsive to carbamazepine treatment, those with the AA genotype for rs3812718 or rs1813502 shown no significant difference in their blood concentration of carbamazepine compared with those with the GG+GA genotype (t=1.562, P=0.125; t=0.843, P=0.562, respectively). rs3812718 and rs1813502 were not in strong linkage disequilibrium.@*CONCLUSION@#Polymorphisms of rs3812718 of the SCN1A gene is associated with carbamazepine resistance among ethnic Zhuang Chinese epilepsy patients from Baise region.

Relationship between the single nucleotide polymorphisms of SCN1A genes and the therapeutic effects of carbamazepine in Zhuang population with epilepsies / 实用医学杂志

Objective To investigate the relationship between the single nucleotide polymorphisms of SCN1A genes and the therapeutic effects of carbamazepine in Zhuang population with epilepsies. Methods We used Mass ARRAY-IPLEX and matrix assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS) technology to detect the SCN1A gene rs4667869 and rs10497275 genotypes in peripheral blood of186 Zhuang individuals with epileptic (66 cases in effective group and 120 cases of ineffective group) who received the standardized treatment of carbamazepine in Baise Region. The reversed phase high-performance liquid chromatography was used to determine blood drug level of carbamazepine. The correlations between the genotypes, alleles and the carbamazepine efficacy of the two groups were evaluated, respectively. We also analyzed the difference of carbamazepine's blood concentration between different genotypes. Results Three genotypes of GG, GC and CC were detected in rs4667869 locus. There were 3 genotypes of GG, GA and AA found in rs 10497275 locus.The differences in the allele distribution (χ2 = 11.790, P = 0.001) and genotype distribution (χ2= 10.655, P =0.005) of the rs4667869 locus were statistically significant between the two groups (ineffective group vs. effective group). However, there was no significant difference in allele distribution (χ2 = 3.335, P= 0.068) and genotype (χ2= 3.046, P = 0.218) for rs 10497275 locus in these two groups. Compared with the GG + GC genotype, the CC genotype of rs4667869 locus significantly reduced the antiepileptic efficacy of carbamazepine (OR = 2.800, 95％CI : 1.495～5.244). W hile there were no significant differences in blood concentration of genotype CC (t=1.273, P = 0.083) comparing with genotypes GG + GC in rs4667869. No significant differences were found in blood concentration between genotype AA and genotypes GG + GA of rs 10497275 (t= 0.963, P = 0.064). Conclusions These results suggest that the single nucleotide polymorphisms of rs4667869 in SCN1A genes could be associated with the drug resistance of carbamazepine in Zhuang population with epilepsies.

Clinical application of pulmonary embolism risk stratification with multiple markers / 实用医学杂志

Objective To evaluate the clinical value of coagulation markers(D-dimer,etc.),myocardial injury markers and blood gas analysis in the risk stratification of PE and prognosis of pulmonary embolism(PE) Methods Totally 201 patients with PE were divided into high-risk group(n=52),intermediate-risk group(n=88),low-risk group(n=61),treatment group(n=67)and 38 normal controls and the level of multiple markers was analyzed. Results DD,Fib,NT-proBNP,PT and PO2of PE patients(high-,intermediate- and low-risk group)were statistically different from those of treatment group and normal controls(P<0.05);cTnⅠ,CK-MB, INR,PTA and RI of high- and intermediate-risk group were statistically different(P < 0.05);but CK and SpO2 were not(P>0.05).Conclusions Multiple markers can be used as a clue to screen high-,intermediate-and low-risk PE.It also could be used to distinguish the prognosis of PE.

Utility of procalcitonin,Hs-CRP and D-Dimer for diagnosis of pulmonary embolism / 实用医学杂志

Objective To investigate the value of procalcitonin(PCT),hypersensitive C-reactive protein (Hs-CRP),D-Dimer(DD)in the diagnosis and prognosis judgment of pulmonary embolism(PE). Methods A total of 201 patients were enrolled in the study since the PE diagnosis were made in hospital from JAN. 2013 to DEC. 2016,in which they were divided into high-risk group(n=52),intermediate-risk group(n=88),low-risk group (n = 61),cure-patient group (n = 67) and 36 normal individuals were selected respectively as a blank control group. Results The average of the PCT,Hs-CRP and DD level in the high,intermediate and low-risk group were significant difference comparing with the cure-patient and control group(P<0.05). Conclusions Teh concertration of PCT and Hs-CRP were associated with different risk stratification of PE. Combined with DD , the level of PCT and Hs-CRP can be used to evaluate the risk stratification of PE and help to judge prognosis and guide treatment.

Correlations of gene polymorphism of ACE, APOE and MTFHR with ischemic stroke in population of Zhuang nationality in western Gui / 实用医学杂志

Objective To study the relationship between ischemic stroke and gene polymorphism of an-giotensin-converting enzyme (ACE), apolipoprotein E (APOE) and methylene tetrahydrofolate reductase (MTHFR) among the population of Zhuang nationality in western Gui. Methods We directly sequenced ACE, APOE and MTHFR genes in 149 cases of ischemic stroke and 109 cases of normal people in western Gui. χ2 test was used to measure the relationship between gene polymorphism and ischemic stroke. Hardy-Weinberg genetic equilibrium test was used to evaluate the reliability of these data. Results In the ischemic stroke group, 62 cases, 22 cases and 65 cases carried II genetype, DD genetype and ID genetype in ACE. χ2 test showed no relationship between ACE gene polymorphism and ischemic stroke. In analysis of the polymorpism of APOE in the ischemic stroke and control group, no relationship between APOE gene polymorphism and ischemic stroke was found by χ2 test. MTFHR gene polymorphism was significantly related with ischemic stroke by χ2 test (P = 0.019). Conclusion Polymorphism of gene MTFHR but neither ACE nor APOE is significantly associated with ischemic stroke.

Study on the immunoregulatory and lethal effects of natural killer T cells on melanoma cells in vitro / 国际检验医学杂志

Objective To explore the immunoregulatory and lethal effects of natural killer T lymphocytes(NKTs) in vitro .Meth‐ods The mixed lymphocyte cultured(MLC) system was established ,in which the B16F10‐luc‐G5 cells were set as target cells ,the total lymphocyte cells were set as effector cells .(1)In the experiment on immunoregulatory effects ,NKT lymphocytes or CD4+CD25+ T lymphocytes were set as regulating cells ,there was three groups ,including the NKT group ,CD4+CD25+ T group and pure target cell control group .Otherewise ,the 1640 blank control group was set by only adding RPMI1640 solution .(2)In the ex‐periment on antitumor effects ,the NKT or natural killer(NK) lymphocytes were set as killer cells ,there was three groups ,inclu‐ding the NKT group ,NK group and pure target cell control group .Mixed culturing 24 ,48 and 72 hours ,the bioluminescence of target cells in MCL system was detected by using the in vivo imaging system .Results (1)In the experiment on immunoregulatory effects ,there were statistically significant differences in measured average photon numbers between NKT group ,CD4+ CD25+ T group and the two control groups(P<0 .05) .The statistically significant differences were also found in the NKT group between 24 hours and 72 hours (P<0 .05) .(2)In the experiment on antitumor effects ,there were statistically significant differences in meas‐ured average photon numbers ,when the NKT group and NK group were compared to the pure target cell control group(P<0 .05) . After culturing 24 and 72 hours ,statistically significant differences were found between NKT group and NK group(P<0 .05) .Con‐clusion The NKT cells could inhibit the lethal effects of lymphocyte cells on target cells ,and the inhibitory effects are changed by the length of culturing .Compared with the CD4+CD25+ T lymphocytes ,NKT lymphocytes have strongger regulatory effects .Addi‐tionally ,the NKT cells have lethal effects on target cells ,which might be weaker than that of NK cells .

Expressions of CD147 and MMP-9 in laryngeal squamous cell carcinoma and their significance / 西安交通大学学报(医学版)

Objective To investigate the expressions of CD147 and matrix metalloproteinases-9 (MMP-9)in laryngeal squamous cell carcinoma (LSCC)tissue and their clinical significance.Methods The expressions of CD147 and MMP-9 were analyzed semi-quantitatively by immunohistochemical staining in LSCC and control group tissues.Results ① The positive rate of CD147 was 83.3% (30/36)in LSCC,which was higher than that in laryngeal polyp (33.3%,5/15)and in adjacent normal tissue (16.7%,6/36);it was related to histological grade, clinical stage and lymph node metastasis status (P<0 .0 5 ).② The positive rate of MMP-9 was 7 2 .2% (2 6/3 6 )in LSCC,which was higher than that in laryngeal polyp (13.3%,2/15)and in adjacent normal tissue (5.6%,2/36);it was related to histological grade,T stage,clinical stage and lymph node metastasis status (P<0.05).③ There was a positive correlation between the expressions of CD147 and MMP-9 in LSCC tissue (r=0.721,P=0.000). Conclusion The over-expressions of CD147 and MMP-9 in LSCC may contribute to the development and metastasis of LSCC.

99Tcm-MDP whole body bone scan for the ancillary diagnosis of malignant fibrous histiocytoma of bone / 中华核医学与分子影像杂志

Objective To investigate the usefulness of 99Tcm-MDP whole body bone scan in bone malignant fibrous histiocytoma (BMFH).Methods Fifteen patients (11 males,4 females,age ranged from 23 to 60 years,average age (50.4±12.8) years) who had documented BMFH and underwent 99Tcm-MDP whole body bone scan were retrospectively analyzed.The appearance of increased uptake,decreased uptake or defect in radioactivity on bone was considered as positive.The typical scintigraphic manifestations of BMFH were summarized and compared to other radiological imaging data.Results All 15 patients showed positive results.The lesions involved femoral in 10 cases(66.7％),46.7％(7/15) of which was distal femur.The lesions also involved sacrum,tibia,humerus,radius each in 1 case and multiple lesions in 1 case.Among 27 lesions found,63.0％ (17/27) showed strong increased radioactivity together with reduced or defect area and 37.0％ (10/27) showed strong increased radioactivity only.X-ray found 20 lesions.Twelve cases underwent CT and 7 cases underwent MRI.Abnormal spots showed on CT and MRI were also positive on the whole body bone scan.Conclusions The most common site of BMFH is femur,especially distal femur.BMFH lesions are presented as strong increased radioactivity together with sparse and defect area on bone scan.The whole body bone scan may be an auxiliary examination to evaluate whether there are multiple bone lesions and bone metastasis.

Application of improved local nerve block anesthesia in correction of nasal septum surgery / 中国医师进修杂志

Objective To explore the effect of improved local nerve block anesthesia in correction of nasal septum surgery.Methods One hundred and fifty-one cases of patients with simply received correction of nasal septum surgery were divided into two groups,72 cases with normal local nerve block (normal group),receiving sphenopalatine and anterior ethmoidal nerve block anesthesia; 79 cases with the improved local nerve block (improved group),receiving sphenopalatine,anterior ethmoidal and incisive canal nerve block anesthesia.Two groups of anesthesia success and anesthesia effect were compared.Results The anesthesia success rates of improved group and normal group who were performed correction of nasal septum surgery without removal of the nasal bottom ridge were 100.0％(41/41) and 97.3％ (36/37),there was no significant difference (P＞ 0.05).But for 73 cases who were performed correction of nasal septum surgery with removal of the nasal bottom ridge,the anesthesia success rate in improved group was higher than that in normal group [92.1％(35/38) vs.45.7％(16/35)],there was significant difference (x2 =18.623,P＜ 0.05).Conclusions Based on the distribution characteristics of sensory nerves of the nasal cavity,and combined with endoscopic techniques,the conventional sphenopalatine and anterior ethmoidal nerve block anesthesia is suitable for nasal septum surgery without removal of the nasal bottom ridge and the advanced sphenopalatine,anterior ethmoidal and incisive canal nerve block anesthesia is the first choice for nasal septum surgery with removal of the nasal bottom ridge.

Change of peripheral blood cortisol concentration in patients with hepatitis B virus-related acute-on-chronic liver failure / 中华传染病杂志

Objective To study the profile of peripheral blood cortisol concentration in patients with hepatitis B virus (HBV)-related acute on chronic liver failure (ACLF) and its association with disease severity and predictive value on prognosis.Methods Forty-five patients with HBV-related ACLF,including 15 patients in early-stage,15 in medium stage and 15 in end-stage; and 15 patients with severe chronic hepatitis B (CHB) were also enrolled.Peripheral blood cortisol concentration was tested by chemiluminesence immunoassay.SPSS version 18.0 was used to compare peripheral blood cortisol concentration among different groups and analyze its correlation with prothrombin activity (PTA),total bilirubin (TBil),albumin (Alb),alanine aminotransferase (ALT),aspartate aminotransferase (AST),HBV DNA,international normalized ratio (INR) and model for end-stage liver disease (MELD) scores.The independent risk factors of prognosis in patients with HBV-related ACLF were determined by performing Logistic regression analysis.Results The concentrations of peripheral blood cortisol in severe CHB group,early stage ACLF group,medium stage ACLF group and end-stage ACLF group were (595.6±114.0),(496.0±87.2),(303.9±81.1),and (183.8±71.8) nmol/L,respectively.A decreasing trend was observed and the differences among groups were statistically significant (F =63.93,P ＜ 0.01).Peripheral blood cortisol concentration were significantly different among subgroups of patients with MELD＜30,followed by 30-40 and ＞40(F 9.01,P＜0.01).Peripheral blood cortisol concentration in patients with ACLF was positively correlated with PTA level (r=0.83,P＜0.01),and inversely correlated with TBil (r =-0.34,P＜0.05),MELDscore (r =-0.60,P＜0.01),AST/ALT (r =-0.35,P＜0.05),and INR (r=-0.59,P＜0.01).Association with Alb,ALT,AST and HBV DNA was not observed.According to multivariate Logistic regression analysis,MELD score,cortisol level,Alb and total cholesterol were independent risk factors of prognosis for patients with ACLF.Serum cortisol concentration in survival group of HBV-related ACLF was higher than that in death group,while the death group exhibited a gradually decreasing trend.Conclusions The peripheral blood cortisol concentration decreases in patients with HBV-related ACLF,which is related to the degree of liver dysfunction,disease severity and prognosis of patients.Moreover,the lower level of cortisol concentration indicates poor short-term prognosis in patients with HBV related ACLF.

The application of anatomization of the recurrent laryngeal nerve under the microscope during thyroid surgery / 临床耳鼻咽喉头颈外科杂志

OBJECTIVE@#To discuss the application of anatomization of the recurrent laryngeal nerve under the microscope in thyroid surgery.@*METHOD@#292 patients with different types of thyroid diseases who underwent surgical intervention were discussed by regression analysis. For all patients, the movement of both vocal cords was normal before the surgery by laryngoscope. And routine anatomization of the recurrent laryngeal nerve under the microscope was conducted during thyroid surgery.@*RESULT@#The total number of recurrent laryngeal nerve exposure in this study was 402. Only three of them got temporary injury. None had permanent damage.@*CONCLUSION@#The recurrent laryngeal nerve can be visualized more clearly by dissection under the microscope, which can best avoid the recurrent laryngeal nerve injury. Particularly it is beneficial for the patients who have prior thyroid surgery history or have thyroid gland cancer.

Effect of pyrrolidine dithiocarbamate on expression of nuclear factor-κB and related inflm(m)atory factors in rat Iippocampus after epilepsy / 中华行为医学与脑科学杂志

Objective To investigate the time course of nuclear factor-κB(NF-κB) and the effects of pyrrolidine dithiocarbamate (PDTC) on the expressions of NF-κB,tumor necrosis factor-α (TNF-α) and interleukin1β(IL-1β) in hippocampus after seizures.Methods Epilepsy were induced by [PTZ] through Intraperitoneal injection.Western blotting was used to detect NF-κB p65 expression in nucleus at various experiment groups in different time points( 14d,21 d,28d,35d).Moreover,mRNA and protein expressions of TNF-α and IL-1β in different experiment groups in different time points by Real-time quantitative PCR and ELISA analysis.Results The expression of NF-κB p65 began to increase in the nuclear fraction in 14d,kept rising in 28d and returned to 14d level in 35d after epilepsy seizures,At 14d,21d,28d and 35d,the expressions of NF-κB in PDTC groups ( (0.54 ±0.07),(0.65 ± 0.08 ),(0.78 ± 0.10),(0.78 ± 0.10) ) was significantly lower than those in PTZ groups ((1.20 ±0.11),(1.42 ±0.14),(1.88 ±0.16),(1.25 ±0.10)) (P＜0.01).After epilepsy seizures,the expression of TNF-α 、IL-1β mRNA was increased in PTZ groups( ( 1.34 ±0.13,0.81 ± O.17 ),( 1.64 ±0.17,1.56±0.20),(2.03 ±0.16,1.65 ±0.18),(1.40 ±0.10,1.30 ±0.13) ) than those in NS groups(P＜0.01 ) ;and compared with PTZ groups PDTC significantly decrease the mRNA expressions of TNF-α and IL-1 β in PDTC groups( (0.96 ±0.1,0.57 ±0.07),(1.36 ±0.15,1.09 ±0.18),(1.47 ±0.14,1.25 ±0.16),(1.12 ±0.12,O.85 ± 0.12) ) (P ＜ 0.05 ).The expressions of TN F-α,IL-1β protein were similar in hippocampal by ELISA.Conclusion Seizures induces NF-κB nucleus translocation and promotes the expressions of TNF-ot and IL-1 β in hippocampus and pyrrolidine dithiocarbamate suppresses NF-κB associated inflammatory pathway in epileptic rat hippocampus.

Comparison of three methods for determination of glomerular filtration rate in chronic glomerulonephritis patients / 中华肾脏病杂志

Objective To identify the accurate measurement of glomerular filtration rate (GFR) in chronic glomerulonephritis (CGN)patients. Methods Forty-two patients were enrolled in the study, including 15 females with age from 18 to 73 years old (mean 46 years old) and 27 males with age from 20 to 77 years old (mean 48 years old). The methods used for measuring GFR were classical dual plasma sample clearance method (tGFR), considered to be the gold standard,renal dynamic imaging method (dGFR), 24-hour creatinine clearance method (24hCcr). The difference and correlation amony them were analyzed. When the difference was significant, Pearson correlation and linear regression analysis were further performed. The difference of GFR detected by dGFR between left and right kidney of patients was compared simultaneously. A two-sided P value＜0.05 was considered as statistically significant. Results Either dGFR or 24hCcr was statistically different from tGFR, but had excellent correlation with tGFR, and the coefficient was 0.916 (P=0.000) and 0.957 (P=0.000) respectively. The linear regressions correlation existed and the regression equations were tGFR=0.936 dGFR-4.648 (F=208.941, P=0.000), tGFR =0.887 24hCcr+2.919 (F=376.513, P=0.000) respectively. Difference had not statistically significance between left and right kidney of patients (P=0.591). Conclusions Neither dGFR nor 24hCcr can substitute tGFR, but both can reflect the GFR of the CGN patients safely and effectively. The decrease of GFR is homochronous for left and right kidney of CGN patients. Therefore, the 24hCcr can be chosen to evaluate the GFR in the hospitals without SPECT.

Clinical significance of cerebrospinal fluid neuropeptide Y in epileptic patients with intelligence disturbance / 中国综合临床

Objective To explore the clinical significance of cerebrospinal fluid neuropeptide Y in epileptic patients with intelligence disturbance.Methods The Full Intelligence Quotient (FIQ) of 78 cases of epileptic patients were assessed by WAIS-RC.The subjects were divided into intelligence disturbance group and non-intelligence disturbance group.Their cerebrospinal fluid neuropeptide Y Was tested by using radio immunoassay.Results The content of neuropeptide Y in intelligence disturbance group Was obviously higher than that in non-intelligence disturbance group (P<0.01).Conclusion Intelligence disturbance in epileptic patients is related to the increased Neuropeptide Y.Neuropeptide Y may reflect the intelligence condition in epileptic patients.

Clinical study of Danshen Shuizi Capsule in preventing restenosis after stent placement intervention / 中华中医药杂志

Objective:To evaluate the preventive effect of Danshen Shuizi Capsule on vascular restenosis after stent implantation.Methods:48 patients with endovascular stent implantation were randomly assigned to control group(n=25) (treated with western medicines)and treatment group(n=23)(treated with Danshen Shuizi Capsule)after endovascular stenting implantation.The intraluminal diameter of stenting segment was measured by QCA at 6 months after stent implantation.Results: The diameter of reference vessel in two groups was not significantly different.The intraluminal diameter of stent segment in the treatment group was not larger than that in the control group(2.22?0.21)mm vs(2.18?0.25)mm.The index number of vessel diameter reduction in treatment group was not less than that in the control group(0.50?0.16)mm vs(0.51?0.11)mm.The rate of restenosis(18.4%)and reintervention(13.1%)in treatment group were not lower than those in the control(20%,12%).However,the complication in treatment group was significantly lower than that in control group.Conclusion:These findings indicated that endovascular was useful for the prevention of endovascular restenosis after stent implantation with fewer complications

Relationship between recovery of spinal function and decompression with internal fixation types in patients with thoracolumbar burst fracture and paraplegia / 中国组织工程研究

BACKGROUND: The most dangerous consequence of thoracolumbar burst fracture is its neurological deficits(caused by primary and/or secondary injury) . It is reported that posterior spinal decompression combined with internal fixation are viable in reduction of fracture and indirect decompression of obstructed spinal canal.OBJECTIVE: To evaluate the effect of anterior decompression combined with STRYKER thoracoluambar anterior internal fixation system in improving spinal canal stenosis and spinal mechanical axis in thoracolumbar burst fracture.DESIGN:It is a case report.SETTING:An orthopedic department of a municipal hospital.PARTICIPANTS:In this study, five patients (four male, one female, aged 17 - 34 years) were recruited who suffered from thoracolumbar burst fracture and spinal cord dysfunction and were treated in the Orthopaedic Departnent of Dongguan Taiping People' s Hospital from September 2001 to February 2002.METHODS: All patients received anterior decompression and intervertebral grafting with iliac or rib bone flap combined with reduction and internal fixation by STRYKER system. X-rays of thoracolumbar vertebrae were taken in the 1st, 3rd, 5th and 9th post-operative months for observation of fusion of injured segments, improvement of narrowed spinal canal and kyphosis angle. And Frankel scale was adopted for evaluation of the patients during follow-up(A for bad and E for excellent).narrowed spinal canal.RESULTS: All of the 5 patients entered analytic stage and follow-up lasted grade B in one patient, all the others got Frankel grade E and could walk gical operation was 68.9% and after operation there was no spinal canal tion to 0° - 6° afterward.CONCLUSION: Anterior spinal decompression can directly and completely provide satisfactory environment for the recovery of neurological of deficits spinal cord. STRYKER thoracoluambar anterior internal fixation system applied in patients with thoracolumbar burst fracture and paraplegia can effectively restore normal spinal curve with the advantages of simple procedure and stable fixation as well as the characteristics of biological internal fixation such as satisfactory reduction and stable fixation.

Content Determination of Danazol in the Anti-hyperplasia of Mammary Glands Cream by UV Spec-trophotometry / 中国药房

OBJECTIVE:To establish a method for the assaying of danazol in anti-hyperplasia of mammary glands cream.METHODS:The UV-spectrophometry was adopted to determine the content of dianozol with the wavelength at285nm.RESULTS:The dianzol was in linear relationship within the concentration range of5～25?g/ml(r=0.9999).The average recovery rate was99.2%(RSD=0.32%,n=5).CONCLUSION:The method is simple,rapid and accurate,which is suitable for the content determination and quality control of the preparation.

Airway mucus,mucins and their secretory regulations / 中国病理生理杂志

Mucus secreted mainly by epithelial goblet cells and submucosal glands covering the respiratory tract plays an important role in the protection from external aggressions, such as solid particles, pathogens and chemical agents by mucociliary clearance. The viscoelastic properties of mucus are mainly determined by the presence of extensively-glycosylated high molecular weight mucins. A lot of factors influence the expression and secretion of mucins in airway, lead to mucus overproduction, which is a distinguishing feature of chronic obstructive pulmonary disease (COPD) and causes disruption of the mucociliary clearance function, resulting in airway block, chronic infection and death.